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BD基质胶/基质膜cornning 356234

简要描述:BD基质胶/基质膜cornning 356234
本公司提供的BD产品货号:354230 354234 356230 356231 356234 356235 356237

  • 产品型号:356230 356231
  • 厂商性质:经销商
  • 更新时间:2023-09-07
  • 访 问 量:622

详细介绍

货号 354248 规格 10ml
供货周期 现货 应用领域 生物产业
BD采用*利技术,从富含胞外基质蛋白的EHS小鼠肿瘤中分离出BDMatrigel基底膜基质,其主要成分由层粘连蛋白,Ⅳ型胶原,巢蛋白,硫酸肝素糖蛋白等组成,还包含生长因子和基质金属蛋白酶等。BDMatrigel基底膜基质在室温条件下,聚合形成具有生物学活性的三维基质,模拟体内细胞基底膜的结构、组成、物理特性和功能,有利于体外细胞的培养和分化,以及对细胞形态、生化功能、迁移、侵染和基因表达的研究。

BDMatrigel基底膜基质形成的三维培养基质,可促进上皮细胞、肝细胞、Sertoli细胞、黑色素瘤细胞、血管内皮细胞、甲状腺细胞及毛囊细胞等的贴壁与分化。同时,Matrigel还能影响乳腺上皮细胞的蛋白表达,支持外周神经的新生和牛输卵管上皮细胞的分化。高浓度的Matrigel适用于研究体内血管生成和肿瘤细胞迁移及肿瘤模型的建立等。

BD基质胶/基质膜cornning356234特点:

Matrigel基质会有色差变化(淡黄色到深红色),是由于酚红和碳酸氢盐与CO2作用引起的,但是与5%CO2平衡后色差即会减少。冻融后,轻轻摇晃试剂瓶使Matrigel分散均匀。所有操作均需在无菌环境下进行,试剂瓶瓶盖可用70%乙醇擦拭,并自然干燥。应使用预冷的移液器以保证Matrigel呈匀浆状。

细胞可在0.5mm厚度的Matrigel基质层表面生长,也可在1mm厚度的Matrigel三维基质内生长。过度稀释的Matrigel会形成非胶质的蛋白层,可以用于细胞贴壁,但不能用于细胞的分化研究。

注意:可将冻融后的Matrigel分装在多个小管,所有分装均需用预冷的冻存管,迅速冷冻并保存,避免多次冻融。

Matrigel在22-35℃温度环境下快速成胶,因此溶解时在4℃冰上过夜冻融(4度时会随着温度的上升部分成胶)。所有用品在使用前需置于冰浴,必须使用预冷的移液管、吸头及小管操作Matrigel。成胶后的Matrigel可以在4℃24-48小时后重新呈液态。

推荐包被与成胶方法:

注意:为了保证Matrigel基质膜的成胶性能与稳定性,稀释浓度不应低于1:3,可用无血清培养基稀释,Matrigel成胶后立即使用。

薄胶成胶方法:

1.冻融后,用预冷的移液枪头混匀Matrigel基质成匀浆状。

2.将需要使用的培养板置于冰上,加入浓度为50μL/cm2生长面积的Matrigel基质。

3.在37℃放置30分钟,即可使用。

厚胶成胶方法:

1.冻融后,用预冷的移液枪头混匀Matrigel基质成匀浆状。

2.将需要使用的培养板置于冰浴,将培养的细胞与Matrigel基质混合,用移液枪头使其悬浮于基质中。加入浓度为150-200μL/cm2生长面积的Matrigel基质。

3.在37℃放置30分钟,可成胶。可以加入细胞培养的基质,也可使细胞直接生长在胶表面。

薄层包被方法:

1.冻融后,用预冷的移液枪头混匀Matrigel基质成匀浆状。

2.根据使用需要,采用无血清培养基稀释Matrigel基质。根据实验需要确定*包被浓度。

3.将稀释的Matrigel基质包被于所需的培养器皿中,包被量至少覆盖整个器皿的生长表面。室温下孵育1小时。

4.去除未结合的Matrigel,用无血清培养基轻轻地冲洗。

本公司提供BD基质胶/基质膜cornning356234354248354230,如有需要欢迎来咨询!

351130Falcon96WellMultiwellInsertSystemwith1.0µmPoreTranslucentPolyester(PET)Membrane,withFeederTrayandLid,1/Case

351131Falcon96WellMultiwellInsertSystemwith1.0µmPoreTranslucentPolyester(PET)Membrane,withFeederTrayandLid,5/Case

351155CorningFluoroBlokHTS24WellMultiwellPermeableSupportSystemwith3.0µmHighDensityPolyester(PET)Membrane,Sterile,1/Pk,1/Cs

351156CorningFluoroBlokHTS24WellMultiwellPermeableSupportSystemwith3.0µmHighDensityPolyester(PET)Membrane,Sterile,5/Pk,5/Cs

351157CorningFluoroBlokHTS24WellMultiwellPermeableSupportSystemwith8.0µmHighDensityPolyester(PET)Membrane,Sterile,1/Pk,1/Cs

351158CorningFluoroBlokHTS24WellMultiwellPermeableSupportSystemwith8.0µmHighDensityPolyester(PET)Membrane,Sterile,5/Pk,5/Cs

351161CorningFluoroBlokHTS96WellMultiwellPermeableSupportSystemwith3.0µmHighDensityPolyester(PET)Membrane,Sterile,1/Pk,1/Cs

351162CorningFluoroBlokHTS96WellMultiwellPermeableSupportSystemwith3.0µmHighDensityPolyester(PET)Membrane,Sterile,5/Pk,5/Cs

351163CorningFluoroBlokHTS96WellMultiwellPermeableSupportSystemwith8.0µmHighDensityPolyester(PET)Membrane,Sterile,1/Pk,1/Cs

351164CorningFluoroBlokHTS96WellMultiwellPermeableSupportSystemwith8.0µmHighDensityPolyester(PET)Membrane,Sterile,5/Pk,5/Cs

351181Falcon24WellMultiwellInsertSystemwith1.0µmPoreHighDensityPolyester(PET)Membrane,5/Case

351183FalconHTS24WellMultiwellPermeableSupportSystemwith3.0µmHighDensityPolyester(PET)Membrane,Sterile,1/Pack,5/Case

351184FalconHTS24WellMultiwellPermeableSupportSystemwith8.0µmHighDensityPolyester(PET)Membrane,Sterile,1/Pack,1/Case

351185FalconHTS24WellMultiwellPermeableSupportSystemwith8.0µmHighDensityPolyester(PET)Membrane,Sterile,1/Pack,5/Case

351186Falcon24WellPolystyreneFeederTray,withLid,Sterile,5/Pack,5/Case

353015CorningGentestPre-coatedPAMPAPlateSystem,withLid,5/Case

353137CorningCELLineDisposableBioreactor

353924Falcon96WellFeederPolystyreneTray,withLid,Sterile,5/Pack,5/Case

353925Falcon®96SquareWellAngledBottomNotTreatedMultiwellInsertSystem,withLid,Sterile,5/Case

353928Falcon96SquareWellFlatBottomMicroplate,withLid,Sterile,5/Pack,5/Case

353938Falcon96SquareWellAngledBottomInsertPlate,withLid,Sterile,5/Case

354001Corning®EpidermalGrowthFactor(EGF),MouseNatural(CultureGrade),100µg,1/Pack

354008Corning®Fibronectin,Human,1mg

354009Corning®7SNerveGrowthFactors(NGF),MouseNatural,100µg,1/Pack

354010Corning®EpidermalGrowthFactor(EGF),MouseNatural(ReceptorGrade),100µg,1/Pack

354022CorningBioCoatPoly-D-Lysine1536WellBlackFlatBottomMicroplate,5/Case

354037Corning®Insulin-likeGrowthFactor(IGF-I),HumanRecombinant(CultureGrade),10µg,1/Pack

354039Corning®TransformingGrowthFactor-b(TGF-b),HumanNatural,1µg,1/Pack

354043Corning®Interleukin-2(IL-2),HumanRecombinant,10,000BRMPUnits,1/Pack

354048Corning®Granulocyte-MacrophageColonyStimulatingFactors(GM-CSF),HumanRecombinant,1µg,1/Pack

354051Corning®Platelet-DerivedGrowthFactor-BB(PDGF-BB),HumanRecombinant,10µg,1/Pack

354052Corning®EpidermalGrowthFactor(EGF),HumanRecombinant,100µg,1/Pack

354058Corning®Interleukin-3(IL-3),MouseRecombinant,10µg,1/Pack

354060Corning®BasicFibroblastGrowthFactors(bFGF),HumanRecombinant,10µg,1/Pack

354066Corning®TumorNecrosisFactor-a(TNF-a),HumanRecombinant,10µg,1/Pack

354068Corning®Interleukin-4(IL-4),HumanRecombinant,5µg,1/Pack

354077Corning®BioCoat™Poly-D-Lysine35mmCoverslipBottomTC-TreatedDishes,20/Pack,20/Case,Nonsterile

354085Corning®BioCoat™Poly-L-Lysine12mm#1GermanGlassCoverslip,40/Pack,80/Case

354086Corning®Biocoat™Poly-D-Lysine12mm#1GermanGlassCoverslip,BulkPacked,80/Case

354087Corning®BioCoat™Poly-D-Lysine/Laminin12mm#1GermanGlassCoverslip,BulkPacked,80/Case

354088Corning®BioCoat™Fibronectin22mmRound#1GermanGlassCoverslip,BulkPacked,60/Case

354089Corning®BioCoat™CollagenI22mmRound#1GermanGlassCoverslip,BulkPacked,60/Case

354105Corning®StemCellFactor(SCF),HumanRecombinant,10µg,1/Pack

354107Corning®VascularEndothelialGrowthFactor(VEGF),HumanRecombinant,10µg,1/Pack

354115Corning®T-CellCultureSupplementwithConA(IL-2CultureSupplement),Rat,100mL,1/Pack

354141CorningBioCoatHTS24WellMultiwellAngiogenesisSystemforEndothelialCellInvasion,1/Pack,1/Case

354142CorningBioCoatHTS24WellMultiwellAngiogenesisSystemforEndothelialCellInvasion,1/Pack,5/Case

354143CorningBioCoatBioCoatFibronectinHTS24WellMultiwellAngiogenesisSystemwith3.0µmMembrane,1/Case

354144CorningBioCoatFibronectinHTS24WellMultiwellAngiogenesisSystemwith3.0µmMembrane,1/Pack,5/Case

354147CorningBioCoatFibronectinHTS96WellMultiwellAngiogenesisSystemwith3.0µmMembrane,1/Pack,1/Case

354148CorningBioCoatFibronectinHTS96WellMultiwellAngiogenesisSystemwith3.0µmMembrane,1/Pack,5/Case

354149CorningBioCoatAngiogenesisSystem:EndothelialCellTubeFormation,96WellBlackMultiwellPlate,1/Case

354150CorningBioCoatAngiogenesisSystem:EndothelialCellTubeFormation,96WellBlackMultiwellPlate,5/Case

354151CorningHumanUmbilicalVeinEndothelialCells,HUVEC-2Cells(>5x105Cells),1Cryovial/Pack

354165CorningBioCoatMatrigelTumorInvasionFluoroBlok24WellPlate,withLid,1/Pack,1/Case

354166CorningBioCoatMatrigelTumorInvasionFluoroBlok24WellPlate,withLid,1/Pack,5/Case

354167CorningBioCoatMatrigelTumorInvasionFluoroBlok96WellPlate,withLid,1/Pack,1/Case

354168CorningBioCoatMatrigelTumorInvasionFluoroBlok96WellPlate,withLid,1/Pack,5/Case

354210Corning®Poly-D-Lysine,20mg

354216CorningCalceinAMFluorescentDye,500µg,10x50µg

354217CorningCalceinAMFluorescentDye,1mg

354218CorningDiIC12(3)FluorescentDye,100mg

354221Corning®rLaminin-521,Human,100µgVial

354222Corning®rLaminin-521,Human,1mg(10x100µgVials)

354223Corning®rLaminin-521,Human,5mg(50x100µgVials)

354230Corning®Matrigel®GrowthFactorReduced(GFR)BasementMembraneMatrix,*LDEV-Free,10mL

354232Corning®Laminin,Mouse,1mg

354233Corning®CollagenIV,Mouse,1mg

354234Corning®Matrigel®BasementMembraneMatrix,*LDEV-Free,10mL

354235Corning®Dispase,100mL

354236Corning®CollagenI,RatTail,100mg

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