| 货号 | 354248 | 规格 | 10ml |
|---|
| 供货周期 | 现货 | 应用领域 | 生物产业 |
|---|
BD采用*利技术，从富含胞外基质蛋白的EHS小鼠肿瘤中分离出BDMatrigel基底膜基质，其主要成分由层粘连蛋白，Ⅳ型胶原，巢蛋白，硫酸肝素糖蛋白等组成，还包含生长因子和基质金属蛋白酶等。BDMatrigel基底膜基质在室温条件下，聚合形成具有生物学活性的三维基质，模拟体内细胞基底膜的结构、组成、物理特性和功能，有利于体外细胞的培养和分化，以及对细胞形态、生化功能、迁移、侵染和基因表达的研究。
BDMatrigel基底膜基质形成的三维培养基质，可促进上皮细胞、肝细胞、Sertoli细胞、黑色素瘤细胞、血管内皮细胞、甲状腺细胞及毛囊细胞等的贴壁与分化。同时，Matrigel还能影响乳腺上皮细胞的蛋白表达，支持外周神经的新生和牛输卵管上皮细胞的分化。高浓度的Matrigel适用于研究体内血管生成和肿瘤细胞迁移及肿瘤模型的建立等。
BD基质胶/基质膜cornning356234特点：
Matrigel基质会有色差变化（淡黄色到深红色），是由于酚红和碳酸氢盐与CO2作用引起的，但是与5％CO2平衡后色差即会减少。冻融后，轻轻摇晃试剂瓶使Matrigel分散均匀。所有操作均需在无菌环境下进行，试剂瓶瓶盖可用70％乙醇擦拭，并自然干燥。应使用预冷的移液器以保证Matrigel呈匀浆状。
细胞可在0.5mm厚度的Matrigel基质层表面生长，也可在1mm厚度的Matrigel三维基质内生长。过度稀释的Matrigel会形成非胶质的蛋白层，可以用于细胞贴壁，但不能用于细胞的分化研究。
注意：可将冻融后的Matrigel分装在多个小管，所有分装均需用预冷的冻存管，迅速冷冻并保存，避免多次冻融。
Matrigel在22-35℃温度环境下快速成胶，因此溶解时在4℃冰上过夜冻融（4度时会随着温度的上升部分成胶）。所有用品在使用前需置于冰浴，必须使用预冷的移液管、吸头及小管操作Matrigel。成胶后的Matrigel可以在4℃24－48小时后重新呈液态。
推荐包被与成胶方法：
注意：为了保证Matrigel基质膜的成胶性能与稳定性，稀释浓度不应低于1：3，可用无血清培养基稀释，Matrigel成胶后立即使用。
薄胶成胶方法：
1.冻融后，用预冷的移液枪头混匀Matrigel基质成匀浆状。
2.将需要使用的培养板置于冰上，加入浓度为50μL/cm2生长面积的Matrigel基质。
3.在37℃放置30分钟，即可使用。
厚胶成胶方法：
1.冻融后，用预冷的移液枪头混匀Matrigel基质成匀浆状。
2.将需要使用的培养板置于冰浴，将培养的细胞与Matrigel基质混合，用移液枪头使其悬浮于基质中。加入浓度为150－200μL/cm2生长面积的Matrigel基质。
3.在37℃放置30分钟，可成胶。可以加入细胞培养的基质，也可使细胞直接生长在胶表面。
薄层包被方法：
1.冻融后，用预冷的移液枪头混匀Matrigel基质成匀浆状。
2.根据使用需要，采用无血清培养基稀释Matrigel基质。根据实验需要确定*包被浓度。
3.将稀释的Matrigel基质包被于所需的培养器皿中，包被量至少覆盖整个器皿的生长表面。室温下孵育1小时。
4.去除未结合的Matrigel，用无血清培养基轻轻地冲洗。
本公司提供BD基质胶/基质膜cornning356234354248354230，如有需要欢迎来咨询！
351130Falcon96WellMultiwellInsertSystemwith1.0µmPoreTranslucentPolyester(PET)Membrane,withFeederTrayandLid,1/Case
351131Falcon96WellMultiwellInsertSystemwith1.0µmPoreTranslucentPolyester(PET)Membrane,withFeederTrayandLid,5/Case
351155CorningFluoroBlokHTS24WellMultiwellPermeableSupportSystemwith3.0µmHighDensityPolyester(PET)Membrane,Sterile,1/Pk,1/Cs
351156CorningFluoroBlokHTS24WellMultiwellPermeableSupportSystemwith3.0µmHighDensityPolyester(PET)Membrane,Sterile,5/Pk,5/Cs
351157CorningFluoroBlokHTS24WellMultiwellPermeableSupportSystemwith8.0µmHighDensityPolyester(PET)Membrane,Sterile,1/Pk,1/Cs
351158CorningFluoroBlokHTS24WellMultiwellPermeableSupportSystemwith8.0µmHighDensityPolyester(PET)Membrane,Sterile,5/Pk,5/Cs
351161CorningFluoroBlokHTS96WellMultiwellPermeableSupportSystemwith3.0µmHighDensityPolyester(PET)Membrane,Sterile,1/Pk,1/Cs
351162CorningFluoroBlokHTS96WellMultiwellPermeableSupportSystemwith3.0µmHighDensityPolyester(PET)Membrane,Sterile,5/Pk,5/Cs
351163CorningFluoroBlokHTS96WellMultiwellPermeableSupportSystemwith8.0µmHighDensityPolyester(PET)Membrane,Sterile,1/Pk,1/Cs
351164CorningFluoroBlokHTS96WellMultiwellPermeableSupportSystemwith8.0µmHighDensityPolyester(PET)Membrane,Sterile,5/Pk,5/Cs
351181Falcon24WellMultiwellInsertSystemwith1.0µmPoreHighDensityPolyester(PET)Membrane,5/Case
351183FalconHTS24WellMultiwellPermeableSupportSystemwith3.0µmHighDensityPolyester(PET)Membrane,Sterile,1/Pack,5/Case
351184FalconHTS24WellMultiwellPermeableSupportSystemwith8.0µmHighDensityPolyester(PET)Membrane,Sterile,1/Pack,1/Case
351185FalconHTS24WellMultiwellPermeableSupportSystemwith8.0µmHighDensityPolyester(PET)Membrane,Sterile,1/Pack,5/Case
351186Falcon24WellPolystyreneFeederTray,withLid,Sterile,5/Pack,5/Case
353015CorningGentestPre-coatedPAMPAPlateSystem,withLid,5/Case
353137CorningCELLineDisposableBioreactor
353924Falcon96WellFeederPolystyreneTray,withLid,Sterile,5/Pack,5/Case
353925Falcon®96SquareWellAngledBottomNotTreatedMultiwellInsertSystem,withLid,Sterile,5/Case
353928Falcon96SquareWellFlatBottomMicroplate,withLid,Sterile,5/Pack,5/Case
353938Falcon96SquareWellAngledBottomInsertPlate,withLid,Sterile,5/Case
354001Corning®EpidermalGrowthFactor(EGF),MouseNatural(CultureGrade),100µg,1/Pack
354008Corning®Fibronectin,Human,1mg
354009Corning®7SNerveGrowthFactors(NGF),MouseNatural,100µg,1/Pack
354010Corning®EpidermalGrowthFactor(EGF),MouseNatural(ReceptorGrade),100µg,1/Pack
354022CorningBioCoatPoly-D-Lysine1536WellBlackFlatBottomMicroplate,5/Case
354037Corning®Insulin-likeGrowthFactor(IGF-I),HumanRecombinant(CultureGrade),10µg,1/Pack
354039Corning®TransformingGrowthFactor-b(TGF-b),HumanNatural,1µg,1/Pack
354043Corning®Interleukin-2(IL-2),HumanRecombinant,10,000BRMPUnits,1/Pack
354048Corning®Granulocyte-MacrophageColonyStimulatingFactors(GM-CSF),HumanRecombinant,1µg,1/Pack
354051Corning®Platelet-DerivedGrowthFactor-BB(PDGF-BB),HumanRecombinant,10µg,1/Pack
354052Corning®EpidermalGrowthFactor(EGF),HumanRecombinant,100µg,1/Pack
354058Corning®Interleukin-3(IL-3),MouseRecombinant,10µg,1/Pack
354060Corning®BasicFibroblastGrowthFactors(bFGF),HumanRecombinant,10µg,1/Pack
354066Corning®TumorNecrosisFactor-a(TNF-a),HumanRecombinant,10µg,1/Pack
354068Corning®Interleukin-4(IL-4),HumanRecombinant,5µg,1/Pack
354077Corning®BioCoat™Poly-D-Lysine35mmCoverslipBottomTC-TreatedDishes,20/Pack,20/Case,Nonsterile
354085Corning®BioCoat™Poly-L-Lysine12mm#1GermanGlassCoverslip,40/Pack,80/Case
354086Corning®Biocoat™Poly-D-Lysine12mm#1GermanGlassCoverslip,BulkPacked,80/Case
354087Corning®BioCoat™Poly-D-Lysine/Laminin12mm#1GermanGlassCoverslip,BulkPacked,80/Case
354088Corning®BioCoat™Fibronectin22mmRound#1GermanGlassCoverslip,BulkPacked,60/Case
354089Corning®BioCoat™CollagenI22mmRound#1GermanGlassCoverslip,BulkPacked,60/Case
354105Corning®StemCellFactor(SCF),HumanRecombinant,10µg,1/Pack
354107Corning®VascularEndothelialGrowthFactor(VEGF),HumanRecombinant,10µg,1/Pack
354115Corning®T-CellCultureSupplementwithConA(IL-2CultureSupplement),Rat,100mL,1/Pack
354141CorningBioCoatHTS24WellMultiwellAngiogenesisSystemforEndothelialCellInvasion,1/Pack,1/Case
354142CorningBioCoatHTS24WellMultiwellAngiogenesisSystemforEndothelialCellInvasion,1/Pack,5/Case
354143CorningBioCoatBioCoatFibronectinHTS24WellMultiwellAngiogenesisSystemwith3.0µmMembrane,1/Case
354144CorningBioCoatFibronectinHTS24WellMultiwellAngiogenesisSystemwith3.0µmMembrane,1/Pack,5/Case
354147CorningBioCoatFibronectinHTS96WellMultiwellAngiogenesisSystemwith3.0µmMembrane,1/Pack,1/Case
354148CorningBioCoatFibronectinHTS96WellMultiwellAngiogenesisSystemwith3.0µmMembrane,1/Pack,5/Case
354149CorningBioCoatAngiogenesisSystem:EndothelialCellTubeFormation,96WellBlackMultiwellPlate,1/Case
354150CorningBioCoatAngiogenesisSystem:EndothelialCellTubeFormation,96WellBlackMultiwellPlate,5/Case
354151CorningHumanUmbilicalVeinEndothelialCells,HUVEC-2Cells(>5x105Cells),1Cryovial/Pack
354165CorningBioCoatMatrigelTumorInvasionFluoroBlok24WellPlate,withLid,1/Pack,1/Case
354166CorningBioCoatMatrigelTumorInvasionFluoroBlok24WellPlate,withLid,1/Pack,5/Case
354167CorningBioCoatMatrigelTumorInvasionFluoroBlok96WellPlate,withLid,1/Pack,1/Case
354168CorningBioCoatMatrigelTumorInvasionFluoroBlok96WellPlate,withLid,1/Pack,5/Case
354210Corning®Poly-D-Lysine,20mg
354216CorningCalceinAMFluorescentDye,500µg,10x50µg
354217CorningCalceinAMFluorescentDye,1mg
354218CorningDiIC12(3)FluorescentDye,100mg
354221Corning®rLaminin-521,Human,100µgVial
354222Corning®rLaminin-521,Human,1mg(10x100µgVials)
354223Corning®rLaminin-521,Human,5mg(50x100µgVials)
354230Corning®Matrigel®GrowthFactorReduced(GFR)BasementMembraneMatrix,*LDEV-Free,10mL
354232Corning®Laminin,Mouse,1mg
354233Corning®CollagenIV,Mouse,1mg
354234Corning®Matrigel®BasementMembraneMatrix,*LDEV-Free,10mL
354235Corning®Dispase,100mL
354236Corning®CollagenI,RatTail,100mg
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